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KMID : 0545119960060060451
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 6 p.451 ~ p.455
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Production of Toxin Protein by Recombinant Escherichia coli with a Thermally Inducible Expression System
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HAN, SE JONG
CHANG, HO NAM/CHANG, YONG KEUN/RHIM, SEONG LYUL
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Abstract
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Physiological studies on the expression of Bacillus thuringiensis subsp. tenebrionis (Btt) gene coding for insecticidal protein in recombinant Echerichia coli 537 were carried out to identify optimal culture condition. It was necessary to shift culture temperature form 30 to 42℃ to express the gene. Expression if the Btt toxin gene by recombinant E. coli 537 began within one hour after induction. Complex nitrogen sources increased production of the insecticidal protein. The total insecticidal protein was 0.5 g/l when using yeast extract as a complex nitrogen source. Soybean hydrolysate showed apparently the highest induction efficiency. After induction, the cellular content of the insecticidal protein was 5.4 times higher than it had been before induction. The optimal cultivation strategy was found to grow cells for 7 hours at 30℃ and then 5-8 hours at 42℃. The optimal cultivation pH for the production of insecticidal protein was 6.5. The Btt toxin produced by the recombinant E. coli 537 was found to have the same level of potency against Colorado potato beetle as original toxin.
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